Modeling of Escherichia coli Inactivation by UV Irradiation at Different pH Values in Apple Cider
Abstract:This study examined the effects and interactions of UV light dose (1,800 to 20,331 μJ/cm2) and apple cider pH (2.99 to 4.41) on the inactivation of Escherichia coli ATCC 25922, a surrogate for E. coli O157:H7. A predictive model was developed to relate the log reduction factor of E. coli ATCC 25922 to the UV dose. Bacterial populations for treated and untreated samples were enumerated with the use of nonselective media. The results revealed that UV dose was highly significant in the inactivation of E. coli, whereas pH showed no significant effect at higher UV doses. Doses of 6,500 μJ/cm2 or more were sufficient to achieve a greater than 5-log reduction of E. coli. Experimental inactivation data were fitted adequately by a logistic regression model. UV irradiation is an attractive alternative to conventional methods for reducing bacteria in unpasteurized apple cider.
Document Type: Research Article
Affiliations: 1: Cornell University, Department of Food Science and Technology, New York State Agricultural Experiment Station, Geneva, New York 14456, USA and Facultad de Ciencias Químicas, Universidad Autónoma de Chihuahua, P.O. Box 1542-C, Chihuahua, Chihuahua, México 31320 2: Cornell University, Department of Food Science and Technology, New York State Agricultural Experiment Station, Geneva, New York 14456, USA 3: FPE Inc., Macedon, New York 14502, USA