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Serological Methods and Selective Agars To Enumerate Campylobacter from Broiler Carcasses: Data from Inter- and Intralaboratory Analyses

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Routine analytical means to estimate Campylobacter numbers per milliliter of carcass rinses are needed in high-sample-throughput poultry laboratories. We compared three serological confirmatory tests that were amenable to such a setting when used in conjunction with Campy-Line and Campy-Cefex Campylobacter selective agars. Pre- and post-chlorinated chiller carcass rinse samples were obtained and held on ice, then analyzed 24 h later in two separate laboratories. Presumptive counts on both pre- and postchiller samples from between laboratories on individual agars and between both agars were highly correlated. Agreement among the three serological tests was nearly complete. The use of a premeasured and dried latex anti-Campylobacter antibody agglutination test format was superior to that of either a liquid latex agglutination format or a direct phosphate-buffer microscopic technique in terms of practicality as was the inclusion of an unarmed latex control to detect auto agglutination. A routine procedure for Campylobacter level estimation was suggested. This procedure, when used in conjunction with a serological confirmatory step, should provide processors with a means to assess reductions in numbers per milliliter of carcass rinses versus strictly presence-absence testing.


Document Type: Research Article

Affiliations: 1: Agricultural Research Service, U.S. Department of Agriculture, Russell Research Center, Athens, Georgia 30605, USA 2: Tyson Foods, Inc., Food Safety and Laboratory Services, Wilksboro, North Carolina, USA

Publication date: May 1, 2004

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