Skip to main content

Rapid Detection of Campylobacter jejuni in Chicken Rinse Water by Melting-Peak Analysis of Amplicons in Real-Time Polymerase Chain Reaction

Buy Article:

$37.00 plus tax (Refund Policy)

Abstract:

Five DNA extraction protocols for the detection of Campylobacter spp. by polymerase chain reaction (PCR) were compared. A method involving Triton X-100 produced template DNA of sufficient quality to allow the detection of Campylobacter jejuni at levels of 100 CFU/ml in pure culture. Primers were designed on the basis of the cadF gene sequence. With a SYBR Green I real-time PCR assay, these primers amplified only sequences present in C. jejuni to produce a product with a melting temperature of 81.5°C. None of the strains of Campylobacter coli, Campylobacter lari, or Campylobacter fetus tested produced this product during the PCR assay. Other noncampylobacter species tested were shown not to possess the cadF sequence. The real-time PCR combined with a rapid, simple Triton X-100 DNA extraction protocol made it possible to detect <10 CFU of C. jejuni per ml of chicken rinse within 14 h.

Keywords:

Document Type: Research Article

Affiliations: 1: Department of Food Science, University of Guelph, 43 McGilvray Street, Guelph, Ontario, Canada N1G 2W1 2: Canadian Research Institute for Food Safety, University of Guelph, 43 McGilvray Street, Guelph, Ontario, Canada N1G 2W1

Publication date: 2003-08-01

More about this publication?
  • Access Key
  • Free ContentFree content
  • Partial Free ContentPartial Free content
  • New ContentNew content
  • Open Access ContentOpen access content
  • Partial Open Access ContentPartial Open access content
  • Subscribed ContentSubscribed content
  • Partial Subscribed ContentPartial Subscribed content
  • Free Trial ContentFree trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more