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Inhibition of Nonproteolytic Clostridium botulinum with Lactic Acid Bacteria and Their Bacteriocins at Refrigeration Temperatures

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Nonproteolytic Clostridium botulinum (strains 17B, Beluga, and 202F) was found to be inhibited by Lactobacillus, Lactococcus, Streptococcus, and Pediococcus species in tests by the spot-on-the-lawn simultaneous-antagonism method at 10, 15, and 25°C. C. botulinum 17B was the most resistant strain. Inhibition zone size increased with decreasing incubation temperature. Six strains of Lactobacillus acidophilus and seven strains of bifidobacteria failed to produce an inhibition zone on buffered reinforced clostridium Prussian blue agar seeded with spores of any of the selected C. botulinum strains. C. botulinum 17B was sensitive to 50 to 100 IU of nisin per ml and to 10 to 20 AU of pediocin A per ml.


Document Type: Miscellaneous

Affiliations: 1: Centre for Advanced Food Research, University of Western Sydney, Blacktown Campus, Locked Bag 1797, South Penrith Distribution Centre, New South Wales 1797, Australia 2: Universita`Cattolica del Sacro Cuore, Istituto di Scienze degli Alimenti e della Nutrizione, Via Emilia Parmense, 84-29100 Piacenza, Italy

Publication date: April 1, 2003

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