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Kinetics of Adsorption and Desorption of Aflatoxin B1 by Viable and Nonviable Bacteria

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Abstract:

The reactions involved in the binding (adsorption) and release (desorption) of aflatoxin B1 (AFB1) to and from the surface of bacteria were investigated. Viable and heat-killed Lactobacillus rhamnosus GG, L. rhamnosus LC-705, and Propionibacterium freudenreichii subsp. shermanii JS were incubated in phosphate-buffered saline containing variable concentrations (0.0017 to 13.3 μg/ml) of AFB1. The relationship between the bacterial surface hydrophobicity and the AFB1 adsorption affinity was also investigated. A linear relationship was observed between the specific rate of AFB1 adsorption and the AFB1 concentration for all bacteria. The nature of desorption of adsorbed AFB1 was investigated by repetitive aqueous washes. A linear relationship was observed between the natural log value of the concentration of AFB1 adsorbed and the number of washes for all bacteria studied. The desorption constants were strain-dependent and were lower for heat-killed bacteria than for viable bacteria. Heat treatment appears to alter the surface properties of the bacteria rather than expose new adsorption sites. No correlation was found between the hydrophobicity and the AFB1 adsorption affinity.

Keywords:

Document Type: Research Article

Affiliations: 1: Department of Microbiology, Faculty of Medicine, National University of Singapore, Singapore 117597 2: Department of Clinical Nutrition; Food and Health Research Centre, Mediteknia, University of Kuopio, P.O.B. 1627, FIN-70211 Kuopio, Finland 3: Australian Water Quality Centre, Private Mail Bag 3, Salisbury, South Australia 5108, Australia 4: Department of Clinical Nutrition, Mediteknia, University of Kuopio, P.O.B. 1627, FIN-70211 Kuopio, Finland 5: Department of Biochemistry and Food Chemistry, Vatselankatu 2, 20014-University of Turku, Turku, Finland

Publication date: March 1, 2003

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