Evidence for Escherichia coli O157:H7 Attachment to Water Distribution Pipe Materials by Scanning Electron Microscopy
Abstract:Scanning electron microscopy observation was used to investigate the adhesion of Escherichia coli O157:H7 on water distribution pipe surfaces such as copper and polyethylene plastic at different contact times and storage temperatures. Our results indicated that E. coli cells could easily attach to both surface types after exposures as short as 1 or 4 h at ambient (20°C) and refrigeration temperatures (4°C). Also, we found that copper surfaces have a higher number of attached E. coli cells than plastic surfaces. The number of cells attached to each type of material depended on the nature of the water distribution pipe surfaces and the length of contact time. In addition, the surface energy value of each surface estimated by contact angle measurements using water, ∝-bromonaphthalene, and dimethyl sulfoxide as wetting agents showed that both copper (41.2 megajoules [MJ]·m-2) and plastic (45.8 MJ·m-2) have a low energy surface. In no cases could evidence of extracellular material be observed on surfaces with either exposure condition.
Document Type: Research Article
Affiliations: 1: Agriculture and Agri-Food Canada, Food Research and Development Centre and Canadian Food Inspection Agency, Health of Animal and Food Laboratory, 3600, Casavant Boulevard West, St-Hyacinthe, Québec, Canada J2S 8E3 2: Agriculture and Agri-Food Canada, Food Research and Development Centre, 3600, Casavant Boulevard West, St-Hyacinthe, Québec, Canada J2S 8E3 3: Agriculture and Agri-Food Canada, Food Research and Development Centre and Canadian Food Inspection Agency, Health of Animal and Food Laboratory, 3600, Casavant Boulevard West, St-Hyacinthe, Québec, Canada J2S 8E3
Publication date: December 1, 2002
- IAFP Members with personal subscriptions to JFP Online: To access full-text JFP or JMFT articles, you must sign-in in the upper-right corner using your Ingenta sign-in details (your IAFP Member Login does not apply to this website). The Journal of Food Protection (JFP) is a refereed monthly publication. Each issue contains scientific research and authoritative review articles reporting on a variety of topics in food science pertaining to food safety and quality. The Journal is internationally recognized as the leading publication in the field of food microbiology with a readership exceeding 11,000 scientists from 70 countries. The Journal of Food Protection is indexed in Index Medicus, Current Contents, BIOSIS, PubMed, Medline, and many others.
Print and online subscriptions are available to IAFP Members and institutional subscribers. IAFP Members with a subscription to JFP Online will have access to all available JFP and JMFT content. Online visitors who are not IAFP Members or journal subscribers will be charged on a pay-per-view basis. Membership and subscription information is available at www.foodprotection.org.
- Information for Authors
- Submit a Paper
- Subscribe to this Title
- Membership Information
- Information for Advertisers
- Ingenta Connect is not responsible for the content or availability of external websites