Arcobacter has been shown to be present in numerous different sources, including poultry, water, and humans exhibiting gastroenteritis. The production of a cytolethal distending toxin (CDT) has been
documented in Campylobacter, Helicobacter, and other species. The polymerase chain reaction was used to screen Arcobacter isolates from poultry, cattle, irrigation water, and human diarrhea
for the presence of CDT genes. Cell filtrates and sonic extracts were also tested for CDT-like activity on Chinese Hamster Ovary, HeLa, and Intestinal 407 (INT407) cells in culture. No CDT amplimers were
observed in any of the Arcobacter isolates investigated. However, toxicity to HeLa and INT407 cells was observed and was subsequently analyzed for cell cycle arrest in the presence of the Arcobacter
extracts with flow cytometry. Cells treated with Arcobacter sonic extracts and filtrates exhibited normal cell cycles, suggesting that CDT is not expressed by Arcobacter. Thus, Arcobacter
was shown to produce an entity that was toxic to some cells in culture, but this entity was toxic in a manner different from that of Campylobacter CDT.
Document Type: Research Article
Department of Animal Science, Texas A&M University, 310 Kleberg, College Station, Texas, 77843-2471, USA
Publication date: November 1, 2002
More about this publication?
IAFP Members with personal subscriptions to JFP Online: To access full-text JFP or JMFT articles, you must sign-in in the upper-right corner using your Ingenta sign-in details (your IAFP Member Login does not apply to this website). The Journal of Food Protection (JFP) is a refereed monthly publication. Each issue contains scientific research and authoritative review articles reporting on a variety of topics in food science pertaining to food safety and quality. The Journal is internationally recognized as the leading publication in the field of food microbiology with a readership exceeding 11,000 scientists from 70 countries. The Journal of Food Protection is indexed in Index Medicus, Current Contents, BIOSIS, PubMed, Medline, and many others.
Print and online subscriptions are available to IAFP Members and institutional subscribers. IAFP Members with a subscription to JFP Online will have access to all available JFP and JMFT content. Online visitors who are not IAFP Members or journal subscribers will be charged on a pay-per-view basis. Membership and subscription information is available at www.foodprotection.org.