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Identification of Species and Measurement of Tetrodotoxin in Dried Dressed Fillets of the Puffer Fish, Lagocephalus lunaris

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Abstract:

The toxicity and species of dried dressed fish fillets were investigated. Three of seven dried dressed fish fillets were found to be toxic, with their levels of toxicity ranging from 9 to 18 mouse units per g. The toxin preparation was further purified and identified as tetrodotoxin and anhydrotetrodotoxin by high-performance liquid chromatography and gas chromatography-mass spectroscopy. The species of these fillets could not be distinguished from Lagocephalus lunaris by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis or restriction site and sequence analysis of a polymerase chain reaction amplicon of the cytochrome b gene. These fish fillets were identified as L. lunaris on the basis of their protein patterns and gene characteristics. Furthermore, the toxic samples contained low levels of tetrodotoxin and its derivative.

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Document Type: Research Article

Affiliations: 1: Department of Food Science, National Taiwan Ocean University, 2 Pei-Ning Road, Keelung 202, Taiwan, Republic of China 2: Department of Food Sanitation, Tajen Institute of Technology, Pingtung, Taiwan, Republic of China

Publication date: October 1, 2002

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