Comparison between VIDAS Automatic Enzyme-Linked Fluorescent Immunoassay and Culture Method for Salmonella Recovery from Pork Carcass Sponge Samples
Authors: Yeh, Kuang-Sheng; Tsai, Chin-En; Chen, Shih-Ping; Liao, Chao-Wei
Source: Journal of Food Protection®, Number 10, October 2002, pp. 1527-1673 , pp. 1656-1659(4)
Abstract:VIDAS Salmonella (VIDAS-SLM) is an automated system that uses the enzyme-linked fluorescent assay method to detect Salmonella species. This study evaluated the efficacy of the VIDAS-SLM method in detecting Salmonella species in pork carcass sponge samples gathered from 10 slaughter plants in Taiwan. Two hundred fifty-seven pork carcass sponge samples were screened by the VIDAS-SLM method and by the culture method in parallel. While 18 sponge samples were found to test positive by both methods, the VIDAS-SLM method detected four additional positive samples for which the culture method failed to recover Salmonella. The specificity of the VIDAS-SLM method was found to be 0.98, and its sensitivity was 1.0, since no false-negative results occurred. Artificially inoculated Salmonella at concentrations as low as 5.0 × 100 CFU/ml was detected in the heat-inactivated sponge sample in the presence or absence of 5.0 × 104 CFU of Citrobacter freundii per ml. Thus, the VIDAS-SLM method is a rapid screening method and a potential alternative to the time- and labor-intensive culture method.
Document Type: Research Article
Affiliations: Division of Animal Medicine, Animal Technology Institute Taiwan, P.O. Box 23, Chunan, Miaoli 350, Taiwan
Publication date: October 1, 2002
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