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Direct Detection of Cryptosporidium parvum Oocysts by Immunomagnetic Separation-Polymerase Chain Reaction in Raw Milk
Authors: Di Pinto A.1; Tantillo M.G.2
Source: Journal of Food Protection®, Volume 65, Number 8, 1 August 2002 , pp. 1345-1348(4)
Abstract:
Cryptosporidium parvum is an emerging protozoan parasite responsible for several serious outbreaks of cryptosporidiosis, an enteric infection characterized by severe intestinal distress. This parasite can be transmitted through contaminated water and raw food in the oocyst form, which is resistant to many environmental stresses and food processes. C. parvum is also commonly found on dairy farms and could be transmitted to humans through contaminated raw milk and dairy products. Thus, an immunomagnetic separation-polymerase chain reaction assay for direct detection of C. parvum oocysts in milk was developed. The procedure was able to detect <10 C. parvum oocysts. Thus, it could be used for monitoring milk samples.Language: English
Document Type: Research article
Affiliations: 1: Dipartimento Sanitàe Benessere degli Animali, Facoltà di Medicina Veterinaria, Università degli Studi-Bari, Provinciale per Casamassima, km 3, 70010 Valenzano, Bari, Italy 2: Dipartimento Sanitàe Benessere degli Animali, Facoltà di Medicina Veterinaria, Università degli Studi-Bari, Provinciale per Casamassima, km 3, 70010 Valenzano, Bari, Italy
Publication date: 2002-08-01
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