Authors: Trachoo N.; Frank J.F.

Source: Journal of Food Protection®, Volume 65, Number 7, 1 July 2002 , pp. 1117-1121(5)

Publisher: International Association for Food Protection

Abstract:

Survival of Campylobacter jejuni in mixed-culture biofilms was determined after treatment with chemical sanitizers including chlorine, quaternary ammonia, peracetic acid (PAA), and a PAA/peroctanoic acid mixture (PAA/POA). Biofilm-producing bacteria (gram-positive rods, Y1 and W1) were isolated from chicken house nipple drinkers. A meat plant isolate (Pseudomonas sp.) was also included as a biofilm producer. Two-day-old biofilms grown on polyvinyl chloride (PVC) plastic coupons in R2A broth at 12°C were incubated with 10⁶CFU/ml C. jejuni for 6 h to allow attachment. The coupons were then rinsed and incubated in fresh media for an additional 24 h. C. jejuni-containing biofilms were detached by vortexing with glass beads in modified brucella broth, which was then enumerated for C. jejuni on selective/differential media. The presence of biofilm enhanced (P < 0.01) the attachment and survival of C. jejuni. After the 24-h incubation, only 20 CFU/cm² of C. jejuni were recovered from the control without biofilms compared to 2,500 to 5,000 CFU/cm² in samples with preexisting biofilms. The presence of biofilm microflora decreased (P < 0.01) the effectiveness of sanitizers against C. jejuni. Chlorine was the most effective sanitizer since it completely inactivated C. jejuni in the biofilms after treatment at 50 ppm for 45 s. C. jejuni in biofilms was susceptible to all sanitizers tested but was not completely inactivated by treatment with quaternary ammonia, PAA, or PAA/POA mixture at 50 and 200 ppm for 45 s.

Language: English

Document Type: Research article

Affiliations: 1: Center for Food Safety, Department of Food Science and Technology, University of Georgia, Athens, Georgia 30602-2106, USA

Publication date: 2002-07-01

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