Application of Random Amplified Polymorphic DNA (RAPD) Analysis for Identification of Grouper (Epinephelus guaza), Wreck Fish (Polyprion americanus), and Nile Perch (Lates niloticus) Fillets

Authors: Asensio L.1; González I.2; Fernández A.2; Rodríguez M.A.3; Lobo E.1; Hernández P.E.3; García T.2; Martín R.2

Source: Journal of Food Protection®, Volume 65, Number 2, 1 February 2002 , pp. 432-435(4)

Publisher: International Association for Food Protection

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Abstract:

A random amplified polymorphic DNA (RAPD) method was developed for the specific identification of grouper (Epinephelus guaza), wreck fish (Polyprion americanus), and Nile perch (Lates niloticus) fillets. Using two different reaction primers (S1 and L1), RAPD analysis produced clear fingerprints from which the three fish species could be easily identified. This approach is rapid and reliable and offers the potential to detect fraudulent or unintentional mislabeling of these species in routine seafood authentication analysis.

Language: English

Document Type: Research article

Affiliations: 1: Departamento de Nutrición y Bromatología III (Higiene y Tecnología de los Alimentos), Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain 2: Departamento de Nutrición y Bromatología III (Higiene y Tecnología de los Alimentos), Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain 3: Departamento de Nutrición y Bromatología III (Higiene y Tecnología de los Alimentos), Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain

Publication date: 2002-02-01

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