Comparison of the Compact Dry TC and 3M Petrifilm ACP Dry Sheet Media Methods with the Spiral Plate Method for the Examination of Randomly Selected Foods for Obtaining Aerobic Colony Counts

Authors: Ellis P.; Meldrum R.

Source: Journal of Food Protection®, Volume 65, Number 2, 1 February 2002 , pp. 423-425(3)

Publisher: International Association for Food Protection

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Abstract:

Two hundred thirty-six randomly selected food and milk samples were examined to obtain aerobic colony counts by two dry sheet media methods and a standard Public Health Laboratory Service spiral plate method. Results for 40 samples were outside the limits of detection for one or more of the tested methods and were not considered. (The limits of detection for the spiral plate method were 200 to 1 × 108 CFU/ml for the spiral plate method and 100 to 3 × 106 CFU/ml for the dry sheet media methods.) The remaining 196 sets of results were analyzed further. When the results from the three methods were compared, correlation coefficients were all >0.80 and slopes and intercepts were close to 1.0 and 0.0, respectively. Mean log values and standard deviations were very similar for all three methods. The results were evaluated according to published UK guidelines for ready-to-eat foods sampled at the point of sale, which include a quality acceptability assessment that is based on aerobic colony counts. Eighty-six percent of the comparable results gave the same verdict with regard to acceptability according to the aerobic colony count guidelines. Both dry sheet media methods were comparable to the spiral plate method and can be recommended for the examination of food.

Language: English

Document Type: Research article

Affiliations: 1: Food, Water and Environmental Section, Cardiff Public Health Laboratory, Public Health Laboratory Service in Wales, University Hospital of Wales, Heath Park, Cardiff CF14 4XW, UK

Publication date: 2002-02-01

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    First published in 1937, the Journal of Food Protection®, is a refereed monthly publication. Each issue contains scientific research and authoritative review articles reporting on a variety of topics in food science pertaining to food safety and quality. The Journal is internationally recognized as the leading publication in the field of food microbiology with a readership exceeding 11,000 scientists from 70 countries. The Journal of Food Protection® is indexed in Index Medicus, Current Contents, BIOSIS, PubMed, Medline, and many others.

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