Synergistic Effect of Nisin and Heat Treatment on the Growth of Escherichia coli O157:H7

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A combination of nisin and heat treatment was found to inhibit Escherichia coli O157:H7 effectively. After organisms were heated at 50, 52.5, and 55°C for 5, 10, and 15 min, respectively, nisin was incorporated into the plates of E. coli O157: H7 at 0, 25, 50, and 100 IU/ml. The concentration of 100 IU/ml nisin significantly inhibited the growth of E. coli O157:H7 heated at 50 and 52.5°C for 15 min. Nisin treatment at 100 IU/ml for 6 h resulted in the elimination of E. coli O157:H7 heated at 55°C for 10 and 15 min.


Document Type: Research Article

Affiliations: 1: Department of Environmental Health, Graduate School of Public Health, Seoul National University, Seoul, 110-799m, Korea 2: Department of Biochemistry, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, 103 Seodundong, Suwon, 441-744, Korea

Publication date: February 1, 2002

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