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Detection Limits of β-Lactam Antibiotics in Ewe Milk by Penzym Enzymatic Test

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The Penzym is an enzymatic test widely used for the detection of β-lactam antibiotic residuals in milk. It is a specific method with good sensitivity to this group of antibiotics and enables results to be obtained within a short time. In the present work, the detection limits of 10 β-lactam antibiotics were determined in ewe milk, given the lack of previous studies of the Penzym test in ovine milk. For each antibiotic, eight concentrations were tested on 20 ewe milk samples proceeding from individual ewes (160 analyses per drug). The limits of the Penzym test were determined by means of logistic regression models, as follows: 5μg/kg amoxicillin, 4μg/kg ampicillin, 33μg/kg cloxacillin, 3μg/kg penicillin "G," 43μg/kg ce-phadroxil, 10μg/kg cephalosporin "C," 16μg/kg cephalexin, 900μg/kg cephoperazone, 120μg/kg Ceftiofur, and 77μg/kg cephuroxime. The percentages of positive results for those antibiotics at the maximum residue limit (MRL) concentration established by the European Union (EU) were: 100% (penicillin "G"), 93.3% (ampicillin), 93.3% (cloxacillin), 56.7% (Ceftiofur), and 56.7% (amoxicillin).


Document Type: Research Article

Affiliations: 1: Cátedra de Biofísica, Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, R. P. L. Kreder 2805, 3080 Esperanza, Argentina 2: Departamento de Ciencia Animal, Universidad Politécnica de Valencia, Camino de Vera, 14, 46071 Valencia, España

Publication date: November 1, 2001

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