Enrichment Procedures and Plating Media for Isolation of Yersinia enterocolitica

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Abstract:

A shortened enrichment procedure (25°C for 24 h) was compared with cold enrichment procedures (4°C for 1 to 3 weeks) and direct plating for isolation of Yersinia enterocolitica from commercial ground meat samples. The combined data of all recovery procedures showed that this organism was isolated from 34% of the ground beef samples. The highest isolation rate was 32% for the 4°C/3-week enrichment, followed by 28% for the 4°C/2-week enrichment, 26% for the 25°C/24-h enrichment, 22% for the 4°C/1-week enrichment, and 10% for direct plating. No significant differences (P > 0.05) in isolation rate occurred between the 4°C/3-week, 4°C/2-week, 25°C/24-h, and 4°C/1-week enrichments. The combined data of all recovery procedures showed that Y. enterocolitica was isolated from 64% of ground pork samples. The highest isolation rate was 48% for the 4°C/3-week enrichment, followed by 40% for the 25°C/24-h enrichment, 34% for the 4°C/2-week enrichment, 24% for the 4°C/1week enrichment, and 24% for direct plating. No significant differences (P > 0.05) in isolation rate occurred between the 4°C/3-week, 25°C/24-h, and 4°C/2-week enrichments. During the plating phase of the experiment, the efficiency of a dyecontaining, Yersinia-selective medium (KV202) was compared with that of a commercially available cefsulodin-irgasan-novobiocin medium. Recovery rates were similar for both media. However, KV202 agar differentiated Y. enterocolitica from such contaminating bacteria as Enterobacter, Serratia, and Salmonella by colony morphologic characteristics and color.

Document Type: Research Article

Affiliations: Food Microbiology Laboratory, Department of Animal Sciences and Industry, Kansas State University, Manhattan, Kansas 66506-1600, USA

Publication date: November 1, 2000

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