Isolation and Characterization of Antagonists for the Biocontrol of the Postharvest Wound Pathogen Botrytis cinerea on Strawberry Fruits
Antagonistic bacteria and yeasts were isolated from the epiphytic flora of stored strawberry fruits and evaluated for their ability to protect strawberry fruit wounds after harvest against Botrytis cinerea. Among selected potential antagonists, three strains of Candida reukaufii
(5L3, 10CL4, 10L2) and one strain of Candida pulcherima (10L8) still protected fruit wounds when applied at 103 CFU/wound, reducing lesion or conidiophore development. In the same conditions, two Enterobacteriaceae (10B1, 5B4) highly reduced pathogen development. Strain
5B4 was still highly inhibitory when inoculated at 102 CFU/wound. The six strains applied on fruits did not produce any significant change in color, brightness, and firmness of fruits. The two yeasts, 5L3 and 10L8, and particularly the two bacteria, 5B4 and 10B1, were selected for
further studies. The four antagonists effectively colonized fruit wounds and strongly inhibited spore germination of B. cinerea in vitro. The bacterial cells surrounded the germinating spores of B. cinerea and attachment of 5L3 cells on germinating spores were additionally observed.
Bacterial antagonists, particularly the strain 5B4, multiplied and rapidly used carbohydrates in strawberry fruit juice despite the low pH (pH 3.5). The efficiency of the bacterial antagonists on fruit wounds was related to their growth and nutritional properties.
Document Type: Research Article
Institut National de la Recherche Agronomique, Station de Technologie des Produits Végétaux, Domaine Saint-Paul, Site Agroparc, 84914 Cedex 9, France
Institut National de la Recherche Agronomique, Station de Pathologie Végétale, domaine Saint-Maurice, 84143 Montfavet Cedex 9, France
Publication date: March 1, 2000
More about this publication?
IAFP members must first sign in on the right to access full text articles of JFP
First published in 1937, the Journal of Food Protection®
, is a refereed monthly publication. Each issue contains scientific research and authoritative review articles reporting on a variety of topics in food science pertaining to food safety and quality. The Journal
is internationally recognized as the leading publication in the field of food microbiology with a readership exceeding 11,000 scientists from 70 countries. The Journal of Food Protection®
is indexed in Index Medicus, Current Contents, BIOSIS, PubMed, Medline, and many others.
Print and online subscriptions are available to Members and Institutional subscribers. Online visitors who are not IAFP Members or journal subscribers will be charged on a pay-per-view basis. Information can be obtained by calling +1 800.369.6337; +1 515.276.3344; fax: +1 515.276.8655, E-mail: firstname.lastname@example.org
or Web site: www.foodprotection.org
- Information for Authors
- Submit a Paper
- Subscribe to this Title
- Membership Information
- Information for Advertisers
- ingentaconnect is not responsible for the content or availability of external websites
Open access content
Free trial content