Occurrence of Escherichia coli O157:H7 in Hamburgers Produced in Brazil
Abstract:The occurrence of Escherichia coli O157:H7 was evaluated in 886 samples of hamburgers produced by eight manufacturers in the south and southeast of Brazil from January to September of 1997. The pathogen was not detected in any of the samples analyzed, although 17 (1.9%) showed the presence of E. coli strains, which presented agglutination with O157 antiserum. These strains were not confirmed, since a subsequent identification indicated that they were fermentation positive for sorbitol, incapable of agglutination with the antisera of other commercial brands, and nonproducers of verocytotoxins. A high rate of false-presumptive-positive tests was observed with strains of enterobacteria when isolated by the culture method and when using a commercially purchased enzymatic immunoassay. This high rate of false presumptive positives was likely because the initial screening for sorbitol-negative strains was carried out using phenol red sorbitol 4-methylumbelliferyl-β-D-glucuronide agar, which was shown to be inadequate for this purpose.
Document Type: Research Article
Affiliations: Instituto de Tecnologia de Alimentos–Ital, Campinas (SP), Brazil
Publication date: November 1, 1999
More about this publication?
- IAFP Members with personal subscriptions to JFP Online: To access full-text JFP or JMFT articles, you must sign-in in the upper-right corner using your Ingenta sign-in details (your IAFP Member Login does not apply to this website). The Journal of Food Protection (JFP) is a refereed monthly publication. Each issue contains scientific research and authoritative review articles reporting on a variety of topics in food science pertaining to food safety and quality. The Journal is internationally recognized as the leading publication in the field of food microbiology with a readership exceeding 11,000 scientists from 70 countries. The Journal of Food Protection is indexed in Index Medicus, Current Contents, BIOSIS, PubMed, Medline, and many others.
Print and online subscriptions are available to IAFP Members and institutional subscribers. IAFP Members with a subscription to JFP Online will have access to all available JFP and JMFT content. Online visitors who are not IAFP Members or journal subscribers will be charged on a pay-per-view basis. Membership and subscription information is available at www.foodprotection.org.
- Information for Authors
- Submit a Paper
- Subscribe to this Title
- Membership Information
- Information for Advertisers
- Ingenta Connect is not responsible for the content or availability of external websites