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Evaluation of a Modified Enzymatic Test for the Detection of Tetracyclines in Milk

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Abstract:

The tetracycline galactosidase (TG) test, a new method for the detection of tetracycline residues in raw milk based on the inhibition of β-galactosidase biosynthesis in Escherichia coli, was previously validated with spiked milk samples. It has now been applied to milk from cows treated with oxytetracycline. In view of the occurrence of false positives, related to highly elevated somatic cell counts (>106/ml), the improved TG test was developed, in which a heating step (80°C, 15 min) preceded the original TG test protocol. A good agreement with other assays (Delvotest SP, the Bacillus cereus microtiter test, the LacTek tetracycline milk screening test, the Charm HVS-8100 tetracycline test) as well as with high-pressure liquid chromatography was obtained. No false negatives were observed with reference to the established maximum residue limit for tetracyclines of 100 μg/kg milk.

Document Type: Research Article

Affiliations: 1: Laboratory for Pharmaceutical Microbiology, Department for Pharmaceutical Analysis, University of Ghent, B-9000 Ghent 2: Department for Animal Product Quality and Transformation Technology, Agricultural Research Centre Ghent, DVK-CLO, B-9090 Melle, Belgium

Publication date: June 1, 1999

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