Detection of Bovine Milk Proteins in Soymilk by Western Blotting
Authors: Molina, Elena; Amigo, Lourdes; Ramos, Mercedes
Source: Journal of Food Protection®, Number 12, December 1998, pp. 1597-1697 , pp. 1691-1694(4)
Abstract:A Western blotting method for the detection of whey milk proteins in commercial soymilks was applied to assess the food safety. Soy proteins and milk proteins were separated by SDS-PAGE in PhastSystem equipment. After the electrophoretic separation, immunodetection with anti-bovine α-lactalbumin and anti-bovine β-lactoglobulin antisera was performed. Adulteration with bovine protein in percentages of 0.1 % in soy protein can be detected. Western blotting of bovine α-lactalbumin and bovine β-lactoglobulin was applied to detect adulteration by bovine milk proteins in different soymilks: powdered soymilk and soy infant formulas.
Document Type: Research Article
Affiliations: Instituto de Fermentaciones Industriales (CSIC), C/Juan de la Cierva 3,28006 Madrid, Spain
Publication date: 1998-12-01
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