Proficiency Samples for Quality Assurance in Trichinella Digestion Tests

$37.00 plus tax (Refund Policy)

Buy Article:


A reliable method to produce proficiency samples containing known numbers of Trichinella spiralis cysts for use in quality assurance systems for Trichinella digestion tests was developed and validated. A filtrate containing Trichinella cysts was produced by homogenizing and filtering the muscles of an experimentally infected rat. Using a stereomicroscope and micropipette, intact cysts were removed from the filtrate and were transferred onto an agar substrate to allow accurate counting and subsequent transfer into a sample matrix. The proficiency sample matrix consisted of 20-g balls of lean ground beef and was combined with 80 g of a Trichinella-free muscle tissue to obtain the required 100-g sample weight for the assay. The mean overall larval recovery from 404 proficiency samples was 93.0%. Larval recoveries ≥95, 85, and 75% occurred in 52.4, 84.4, and 94.3%, respectively, of the 404 samples tested. Results indicated that, after a short training period, technicians with no prior experience in digestion techniques performed as well as experienced technicians. The maximum shelf life of proficiency samples was not determined but was at least 3 weeks. Validation data were used to develop panels composed of proficiency samples prepared as described above and to establish guidelines for the interpretation of proficiency panel results.

Document Type: Research Article

Affiliations: Centre for Animal Parasitology, Canadian Food Inspection Agency, 116 Veterinary Road, Saskatoon, Saskatchewan, Canada S7N 2R3

Publication date: October 1, 1998

More about this publication?
  • IAFP members must first sign in on the right to access full text articles of JFP

    First published in 1937, the Journal of Food Protection®, is a refereed monthly publication. Each issue contains scientific research and authoritative review articles reporting on a variety of topics in food science pertaining to food safety and quality. The Journal is internationally recognized as the leading publication in the field of food microbiology with a readership exceeding 11,000 scientists from 70 countries. The Journal of Food Protection® is indexed in Index Medicus, Current Contents, BIOSIS, PubMed, Medline, and many others.

    Print and online subscriptions are available to Members and Institutional subscribers. Online visitors who are not IAFP Members or journal subscribers will be charged on a pay-per-view basis. Information can be obtained by calling +1 800.369.6337; +1 515.276.3344; fax: +1 515.276.8655, E-mail: or Web site:
  • Information for Authors
  • Submit a Paper
  • Subscribe to this Title
  • Membership Information
  • Information for Advertisers
  • ingentaconnect is not responsible for the content or availability of external websites
Related content



Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more