Proficiency Samples for Quality Assurance in Trichinella Digestion Tests
Abstract:A reliable method to produce proficiency samples containing known numbers of Trichinella spiralis cysts for use in quality assurance systems for Trichinella digestion tests was developed and validated. A filtrate containing Trichinella cysts was produced by homogenizing and filtering the muscles of an experimentally infected rat. Using a stereomicroscope and micropipette, intact cysts were removed from the filtrate and were transferred onto an agar substrate to allow accurate counting and subsequent transfer into a sample matrix. The proficiency sample matrix consisted of 20-g balls of lean ground beef and was combined with 80 g of a Trichinella-free muscle tissue to obtain the required 100-g sample weight for the assay. The mean overall larval recovery from 404 proficiency samples was 93.0%. Larval recoveries ≥95, 85, and 75% occurred in 52.4, 84.4, and 94.3%, respectively, of the 404 samples tested. Results indicated that, after a short training period, technicians with no prior experience in digestion techniques performed as well as experienced technicians. The maximum shelf life of proficiency samples was not determined but was at least 3 weeks. Validation data were used to develop panels composed of proficiency samples prepared as described above and to establish guidelines for the interpretation of proficiency panel results.
Document Type: Research Article
Affiliations: Centre for Animal Parasitology, Canadian Food Inspection Agency, 116 Veterinary Road, Saskatoon, Saskatchewan, Canada S7N 2R3
Publication date: October 1, 1998
More about this publication?
- IAFP Members with personal subscriptions to JFP Online: To access full-text JFP or JMFT articles, you must sign-in in the upper-right corner using your Ingenta sign-in details (your IAFP Member Login does not apply to this website). The Journal of Food Protection (JFP) is a refereed monthly publication. Each issue contains scientific research and authoritative review articles reporting on a variety of topics in food science pertaining to food safety and quality. The Journal is internationally recognized as the leading publication in the field of food microbiology with a readership exceeding 11,000 scientists from 70 countries. The Journal of Food Protection is indexed in Index Medicus, Current Contents, BIOSIS, PubMed, Medline, and many others.
Print and online subscriptions are available to IAFP Members and institutional subscribers. IAFP Members with a subscription to JFP Online will have access to all available JFP and JMFT content. Online visitors who are not IAFP Members or journal subscribers will be charged on a pay-per-view basis. Membership and subscription information is available at www.foodprotection.org.
- Information for Authors
- Submit a Paper
- Subscribe to this Title
- Membership Information
- Information for Advertisers
- Ingenta Connect is not responsible for the content or availability of external websites