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Characterization of Staphylococcal Bovine Mastitis Isolates Using the Polymerase Chain Reaction

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A polymerase chain reaction (PCR) assay was adapted to detect toxin genes of staphylococcal isolates from cases of bovine mastitis, Samples were obtained from three geographical areas: Korea and Idaho and Washington in the northwest United States. Samples from Korea and Washington were randomly chosen. Idaho samples were from a prospective study of mastitis etiology. Forty-one milk samples from 25 commercial farms in south-central Idaho were collected from cows with symptoms of mastitis. Although Staphylococcus aureus constituted 37.5% of mastitis isolates, these isolates lacked genes for staphylococcal enterotoxins (SEs), toxic shock syndrome toxin, and exfoliative toxins. In contrast, 4 of 13 isolates from Washington and 6 of 20 isolates from South Korea expressed SEs. These results suggest that PCR may be an effective means of screening bovine isolates for toxins. They also emphasize the potential for significant geographic differences in mastitis etiology.

Document Type: Research Article

Affiliations: 1: Department of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, Idaho 83844, USA 2: Department of Microbiology, College of Veterinary Medicine, Seoul National University, Suwon 441-744, Korea 3: Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99163, USA 4: Department of Veterinary Clinical Medicine and Surgery, Washington State University, Pullman, Washington 99163, USA 5: Department of Animal and Veterinary Sciences, University of Idaho Research and Extension Center, Twin Falls, Idaho 83303-1827, USA

Publication date: October 1, 1998

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