Confirmation of Presumptive Salmonella Colonies by the Polymerase Chain Reaction

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The potential of a genus-specific polymerase chain reaction (PCR) for the confirmation of Salmonella colonies was evaluated on 209 presumptive Salmonella colonies obtained by the standard method ISO 6579. The PCR method employing primers ST11 and ST15 (S. Aabo et al., Mol. Cell. Probes 7:171–178, 1993) gave results identical (100%) to those of the biochemical and serological identification, in terms of discrimination of Salmonella from non-Salmonella strains. PCR could be used directly on the colonies from selective plating media, which allowed a reduction of the time required for confirmation to a maximum of 6 h.

Document Type: Research Article

Affiliations: 1: Department of Microbiology and Chemistry, Food Research Institute, Priemyselná 4, SK-82006 Bratislava, Slovakia 2: State Veterinary Institute, Botanickd 15, SK-84252 Bratislava, Slovakia 3: Agricultural Research Centre, Departmentfor Animal Product Quality, Brusselsesteenweg 370, B-9090 Melle, Belgium

Publication date: October 1, 1998

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