Alteration in Sporulation, Enterotoxin Production, and Protein Synthesis by Clostridium perfringens Type A Following Heat Shock

Authors: Heredia, Norma L.¹; Labbé, Ronald G.²; García-Alvarado, José Santos¹

Source: Journal of Food Protection®, Volume 61, Number 9, September 1998 , pp. 1143-1147(5)

Publisher: International Association for Food Protection

Buy & download fulltext article:

Price: $37.00 plus tax (Refund Policy)

Abstract:

Application of a heat shock (43 to 50°C) applied early during the sporulation process of Clostridium perfringens delayed spore and enterotoxin production. Final levels of heat-resistant spores were similar to the control, but enterotoxin levels were reduced when the heat shock was applied at the third hour of incubation. The response of the microorganism to the heat shock was also examined by analysis of pulse-labeled proteins. Seven heat shock proteins (HSPs) associated with vegetative cells were identified by polyacrylamide gel electrophoresis. Most were localized mainly in the membrane, although one small protein was mostly present in the cytoplasm. Fewer HSPs were detected during sporulation. Two HSPs were immunologically related to the GroEL and DnaK HSPs from Lactobacillus lactis and Escherichia coli, respectively.

Document Type: Research article

Affiliations: 1: Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, Apdo. Postal 124-F, San Nicolás N.L. México 66451 2: Food Science Department, University of Massachusetts, Amherst, Massachusetts 01003, USA

Publication date: 1998-09-01

More about this publication?

IAFP members must first sign in on the right to access full text articles of JFP

First published in 1937, the Journal of Food Protection^®, is a refereed monthly publication. Each issue contains scientific research and authoritative review articles reporting on a variety of topics in food science pertaining to food safety and quality. The Journal is internationally recognized as the leading publication in the field of food microbiology with a readership exceeding 11,000 scientists from 70 countries. The Journal of Food Protection^® is indexed in Index Medicus, Current Contents, BIOSIS, PubMed, Medline, and many others.

Print and online subscriptions are available to Members and Institutional subscribers. Online visitors who are not IAFP Members or journal subscribers will be charged on a pay-per-view basis. Information can be obtained by calling +1 800.369.6337; +1 515.276.3344; fax: +1 515.276.8655, E-mail: info@foodprotection.org or Web site: www.foodprotection.org
Information for Authors
Submit a Paper
Subscribe to this Title
Membership Information
Information for Advertisers
ingentaconnect is not responsible for the content or availability of external websites

Alteration in Sporulation, Enterotoxin Production, and Protein Synthesis by Clostridium perfringens Type A Following Heat Shock

Buy & download fulltext article:

Tools

Key

Text size:

Useful pages