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Gamma Irradiation and Ozone Treatment for Inactivation of Escherichia coli O157:H7 in Culture Media

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A study was conducted to investigate the reduction and elimination of Escherichia coli O157:H7 by the effects of gamma irradiation and ozone treatment. Log phase cells were found to be more sensitive to gamma irradiation than stationary phase cells. E. coli O157:H7 was found to be considerably more resistant to irradiation at −18°C than at 20°C. The D values for this organism for treatment with ozone in tryptic soy agar were higher than those for treatment with ozone in phosphate buffer. Gamma irradiation at a dose of 1.5 kGy or ozone treatment at a concentration of 3 to 18 ppm for 20 to 50 min was required to assure the elimination of E. coli O157:H7.

Document Type: Research Article

Affiliations: 1: Department of Food Irradiation, Korea Atomic Energy Research Institute, Yusung, P.O. Box 105, Taejon, 305-353, Korea 2: Department Food and Nutrition, Chosun University, Kwangju, 501-759, Korea

Publication date: June 1, 1998

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