Skip to main content

Automated Detection of Hydrogen Sulfide Release from Thiosulfate by Salmonella spp.

Buy Article:

$37.00 plus tax (Refund Policy)


Hydrogen sulfide production is used in conventional tests for identification and differentiation of Salmonella spp. from other species of Enterobacteriaceae, and a black precipitate on agar media is the indicator of the reaction. Selective liquid media were formulated for automated optical detection of H2S in salmonellae using the BioSys instrument. The media contained thiosulfate and ferric ammonium citrate, and production of HzS caused copious black pigmentation of the broth. Combination of the H2S indicators with dulcitol or xylose as fermentable carbohydrate, lysine, ornithine or arginine to induce decarboxylase activity, and Tergitol 4 as inhibitor selectively identified six Salmonella spp. by a sharp drop in transmittance at 585 nm. The time for detection of transmittance changes was inversely proportional to initial numbers of CFU in the media: 10 h for 105 CFU/ml and 17 h for 101 CFU/ml. No detection was observed in six non-Salmonella species of Enterobacteriaceae tested.

Document Type: Research Article

Affiliations: Department of Nutrition and Food Science, Wayne State University, Detroit, Michigan48202, USA

Publication date: May 1, 1998

More about this publication?
  • IAFP Members with personal subscriptions to JFP Online: To access full-text JFP or JMFT articles, you must sign-in in the upper-right corner using your Ingenta sign-in details (your IAFP Member Login does not apply to this website).

    The Journal of Food Protection (JFP) is a refereed monthly publication. Each issue contains scientific research and authoritative review articles reporting on a variety of topics in food science pertaining to food safety and quality. The Journal is internationally recognized as the leading publication in the field of food microbiology with a readership exceeding 11,000 scientists from 70 countries. The Journal of Food Protection is indexed in Index Medicus, Current Contents, BIOSIS, PubMed, Medline, and many others.

    Print and online subscriptions are available to IAFP Members and institutional subscribers. IAFP Members with a subscription to JFP Online will have access to all available JFP and JMFT content. Online visitors who are not IAFP Members or journal subscribers will be charged on a pay-per-view basis. Membership and subscription information is available at
  • Information for Authors
  • Submit a Paper
  • Subscribe to this Title
  • Membership Information
  • Information for Advertisers
  • ingentaconnect is not responsible for the content or availability of external websites

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more