Identification and Characterization of Salmonella Isolates by Automated Ribotyping
Author: Oscar, Thomas P.
Source: Journal of Food Protection®, Number 5, May 1998, pp. 513-648 , pp. 519-524(6)
Abstract:A study was conducted with the RiboPrinter, an automated ribotyping system, to evaluate its ability to identify and characterize isolates of Salmonella from broiler operations. Isolates of Salmonella obtained from a local broiler company were serotyped by a reference laboratory and ribotyped using the RiboPrinter. The RiboPrinter generated ribotype patterns by probing EcoRI digests of Salmonella DNA with an E. coli DNA probe to the ribosomal RNA operon. The RiboPrinter identified isolates by band matching of their ribotype patterns to ribotype patterns in its database. In addition, the RiboPrinter characterized isolates by sorting them into ribotypes on the basis of the similarity of their ribotype patterns. Of 117 isolates, the RiboPrinter identified 34 (29%) at the serotype level, 11 (9%) at the strain level, 46 (39%) at the genus level, and 26 (22%) were not identified. Thus, only 38% of the isolates were identified at or below the serotype level, indicating that the RiboPrinter was limited in its ability to identify Salmonella isolates by band matching. In contrast, the RiboPrinter was very effective at characterizing Salmonella isolates. Out of 108 isolates, the RiboPrinter detected 31 ribotypes, compared to serotyping which only detected 22 types of Salmonella. Thus, automated ribotyping was more discriminatory than serotyping. However, when results of both typing methods were combined, 40 types of Salmonella were detected, indicating that the best discrimination was obtained when automated ribotyping and serotyping were used together.
Document Type: Research Article
Affiliations: U.S. Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, Microbial Food Safety Research Unit Worksite, 1124 Trigg Hall, University of Maryland Eastern Shore, Princess Anne, Maryland 21853, USA
Publication date: May 1, 1998
- IAFP members must first sign in on the right to access full text articles of JFP First published in 1937, the Journal of Food Protection®, is a refereed monthly publication. Each issue contains scientific research and authoritative review articles reporting on a variety of topics in food science pertaining to food safety and quality. The Journal is internationally recognized as the leading publication in the field of food microbiology with a readership exceeding 11,000 scientists from 70 countries. The Journal of Food Protection® is indexed in Index Medicus, Current Contents, BIOSIS, PubMed, Medline, and many others.
Print and online subscriptions are available to Members and Institutional subscribers. Online visitors who are not IAFP Members or journal subscribers will be charged on a pay-per-view basis. Information can be obtained by calling +1 800.369.6337; +1 515.276.3344; fax: +1 515.276.8655, E-mail: email@example.com or Web site: www.foodprotection.org
- Information for Authors
- Submit a Paper
- Subscribe to this Title
- Membership Information
- Information for Advertisers
- ingentaconnect is not responsible for the content or availability of external websites