Supplementing Pools of Egg Contents with Broth Culture Media To Improve Rapid Detection of Salmonella enteritidis
Abstract:Culturing egg contents to detect Salmonella enteritidis (SE) has become an important tool for identifying infected laying flocks and thereby reducing the transmission of SE to humans by contaminated eggs. The present study evaluated the efficacy of supplementing incubating egg pools with selective and nonselective enrichment broth media (prepared at higher than usual concentrations) for rapidly isolating SE by a direct plating culture method. When 100-ml pools of liquid whole egg from a mixture of 60 egg contents were contaminated with approximately 10 SE cells each, supplementation with ferrous sulfate or with concentrates of either tryptone soya broth or Rappaport-Vassiliadis broth significantly improved SE recovery. When 100-ml egg-contents pools were contaminated with approximately 2 SE cells each, the addition of concentrated tryptone soya broth to incubating egg pools resulted in significantly better SE recovery than did iron supplementation. Efficient presumptive detection of very low incidences and levels of SE contamination by direct plating was thus accomplished in a total of 48 h by adding concentrated tryptone soya broth to incubating egg pools.
Document Type: Research Article
Affiliations: United States Department of Agriculture, Agricultural Research Service, Southeast Poultry Research Laboratory, 934 College Station Road, Athens, Georgia 30605, USA
Publication date: January 1, 1998
More about this publication?
- IAFP Members with personal subscriptions to JFP Online: To access full-text JFP or JMFT articles, you must sign-in in the upper-right corner using your Ingenta sign-in details (your IAFP Member Login does not apply to this website). The Journal of Food Protection (JFP) is a refereed monthly publication. Each issue contains scientific research and authoritative review articles reporting on a variety of topics in food science pertaining to food safety and quality. The Journal is internationally recognized as the leading publication in the field of food microbiology with a readership exceeding 11,000 scientists from 70 countries. The Journal of Food Protection is indexed in Index Medicus, Current Contents, BIOSIS, PubMed, Medline, and many others.
Print and online subscriptions are available to IAFP Members and institutional subscribers. IAFP Members with a subscription to JFP Online will have access to all available JFP and JMFT content. Online visitors who are not IAFP Members or journal subscribers will be charged on a pay-per-view basis. Membership and subscription information is available at www.foodprotection.org.
- Information for Authors
- Submit a Paper
- Subscribe to this Title
- Membership Information
- Information for Advertisers
- Ingenta Connect is not responsible for the content or availability of external websites