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Combinations of Heat Treatment and Sodium Chloride That Prevent Growth from Spores of Nonproteolytic Clostridlum botulinum

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The ability of spores of nonproteolytic Clostridium botulinum to survive heat treatment and subsequently produce turbidity at 10°C in the presence of NaCl was quantified for different incubation times. Spores of nonproteolytic C. botulinum strain Eklund 17B were heated at 75°C for up to 4 min or 90°C for up to 30 min and subsequently incubated at 10°C in PYGS broth containing 1.5%, 3.0%, or 4.0% NaCl (wt/vol) with or without 10 μg hen egg white lysozyme ml−1. Heat treatment at 90°C for 30 min or incubation at 10°C in the presence of 4.0% NaCl did not prevent growth from up to 1.4 × 105 spores. Heat treatment at 90°C for 15 min and incubation in the presence of 4.0% NaCl did prevent growth in the same conditions.


Document Type: Research Article

Affiliations: Genetics and Microbiology Department, Institute of Food Research, Norwich Laboratory, Norwich Research Park, Colney, Norwich, NR4 7UA, UK

Publication date: December 1, 1997

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