Enterotoxin Production by Staphylococcus warneri CCRC 12929, a Coagulase-Negative strain
Abstract:Production of staphylococcal enterotoxin A (SEA) and enterotoxin D (SED) by Staphylococcus warneri was first examined in three different media including brain heart infusion (BHI) broth, NZ-amine (NZA) medium, and 3 + 3 medium containing 3% NZ-amine A plus 3% Hy-Case Amino. Results of these investigations revealed that S. warneri produced the highest amount of SEA in BHI broth. SED yields were higher when BHI broth or NZA medium was used as the culture medium. On the other hand, SEA and SED production was found to be lowest in 3 + 3 medium. Further study showed that the optimum pH, for both SEA production and SED production was 7.0. Addition of 10% (wt/vol) sodium chloride to BHI broth reduced enterotoxin production by S. warneri. SEA production was reduced and SED production was completely inhibited in the presence of 15% (wt/vol) NaCl. Supplementation of the media with 0.4 or 1.5 mM magnesium sulfate markedly reduced SED production by S. warneri. Reduced production of both SEA and SED was also observed when BHI broth was supplemented with 0.1 M glucose or glycerol.
Document Type: Research Article
Affiliations: Graduate institute of Food Science and Technology, National Taiwan University, 59, Lane 144, Keelung Rd., Sec. 4, Taipei, Taiwan
Publication date: August 1, 1997
More about this publication?
- IAFP Members with personal subscriptions to JFP Online: To access full-text JFP or JMFT articles, you must sign-in in the upper-right corner using your Ingenta sign-in details (your IAFP Member Login does not apply to this website). The Journal of Food Protection (JFP) is a refereed monthly publication. Each issue contains scientific research and authoritative review articles reporting on a variety of topics in food science pertaining to food safety and quality. The Journal is internationally recognized as the leading publication in the field of food microbiology with a readership exceeding 11,000 scientists from 70 countries. The Journal of Food Protection is indexed in Index Medicus, Current Contents, BIOSIS, PubMed, Medline, and many others.
Print and online subscriptions are available to IAFP Members and institutional subscribers. IAFP Members with a subscription to JFP Online will have access to all available JFP and JMFT content. Online visitors who are not IAFP Members or journal subscribers will be charged on a pay-per-view basis. Membership and subscription information is available at www.foodprotection.org.
- Information for Authors
- Submit a Paper
- Subscribe to this Title
- Membership Information
- Information for Advertisers
- Ingenta Connect is not responsible for the content or availability of external websites