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Identification and Differentiation of Coagulase-Negative Staphylococcus aureus by Polymerase Chain Reaction

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Abstract:

Staphylococcus aureus is an etiological agent of a wide variety of human and animal infections. The majority of S. aureus are coagulase-positive; however, some may be atypical in that they do not produce coagulase. Incorrect identification of an isolate can impact implementation of effective treatment and/or control measures. In this study, polymerase chain reaction-based DNA finger-printing was used to differentiate coagulase-positive Staphylococcus aureus (CPSA) from coagulase-negative Staphylococcus aureus (CNSA). A total of 29 CNSA and 50 CPSA were evaluated. PCR-based DNA fingerprinting differentiated CNSA from CPSA on the basis of visible observation and densitometric evaluation. The method is rapid and accurate, eliminating variability associated with conventional techniques.

Keywords: COAGULASE TEST; IDENTIFICATION; POLYMERASE CHAIN REACTION; STAPHYLOCOCCUS AUREUS

Document Type: Research Article

Affiliations: 1: Department of Food Science, Rutgers University, New Brunswick, New Jersey 08903, USA 2: Institute of Agriculture, The University of Tennessee, Knoxville, Tennessee 37901, USA

Publication date: June 1, 1997

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    First published in 1937, the Journal of Food Protection®, is a refereed monthly publication. Each issue contains scientific research and authoritative review articles reporting on a variety of topics in food science pertaining to food safety and quality. The Journal is internationally recognized as the leading publication in the field of food microbiology with a readership exceeding 11,000 scientists from 70 countries. The Journal of Food Protection® is indexed in Index Medicus, Current Contents, BIOSIS, PubMed, Medline, and many others.

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