Staphylococcus aureus is an etiological agent of a wide variety of human and animal infections. The majority of S. aureus are coagulase-positive; however, some may be atypical in that they do not produce coagulase. Incorrect identification of an isolate can impact implementation
of effective treatment and/or control measures. In this study, polymerase chain reaction-based DNA finger-printing was used to differentiate coagulase-positive Staphylococcus aureus (CPSA) from coagulase-negative Staphylococcus aureus (CNSA). A total of 29 CNSA and 50 CPSA were
evaluated. PCR-based DNA fingerprinting differentiated CNSA from CPSA on the basis of visible observation and densitometric evaluation. The method is rapid and accurate, eliminating variability associated with conventional techniques.
Department of Food Science, Rutgers University, New Brunswick, New Jersey 08903, USA 2:
Institute of Agriculture, The University of Tennessee, Knoxville, Tennessee 37901, USA
Publication date: June 1, 1997
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