Monoclonal Antibodies and an Indirect ELISA for Detection of Psychrotrophic Bacteria in Refrigerated Milk
Authors: Gutiérrez, Rosalba; González, Isabel; García, Teresa; Carrera, Ester; Sanz, Bernabé; Hernández, Pablo E.; Martín, Rosario
Source: Journal of Food Protection®, Number 1, January 1997, pp. 6-87 , pp. 23-27(5)
Abstract:Monoclonal antibodies generated against live cells of Pseudomonas jiuorescens have been used in an indirect ELISA format for the detection of Pseudomonas spp. and related psychrotrophic bacteria in refrigerated milk. The immunorecognition of monoclonal antibodies adsorbed to bacteria bound to the wells of a microtiter plate was performed with rabbit anti-mouse immunoglobulins conjugated to horseradish peroxidase. Subsequent enzymic conversion of the substrate resulted in distinct absorbance differences when assaying milk samples containing psychrotrophic bacteria in the range l05 to 109 CFU ml−1. The detection threshold for the ELISA assay developed in this work is 105 CFU ml−1.
Document Type: Research Article
Affiliations: Departamento de Nutrición y Bromatología III, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain
Publication date: 1997-01-01
- IAFP members must first sign in on the right to access full text articles of JFP First published in 1937, the Journal of Food Protection®, is a refereed monthly publication. Each issue contains scientific research and authoritative review articles reporting on a variety of topics in food science pertaining to food safety and quality. The Journal is internationally recognized as the leading publication in the field of food microbiology with a readership exceeding 11,000 scientists from 70 countries. The Journal of Food Protection® is indexed in Index Medicus, Current Contents, BIOSIS, PubMed, Medline, and many others.
Print and online subscriptions are available to Members and Institutional subscribers. Online visitors who are not IAFP Members or journal subscribers will be charged on a pay-per-view basis. Information can be obtained by calling +1 800.369.6337; +1 515.276.3344; fax: +1 515.276.8655, E-mail: firstname.lastname@example.org or Web site: www.foodprotection.org
- Information for Authors
- Submit a Paper
- Subscribe to this Title
- Membership Information
- Information for Advertisers
- ingentaconnect is not responsible for the content or availability of external websites