Whole Blood, Serum, and Tissue Fluids in an Enzyme Immunoassay for Swine Trichinellosis

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Abstract:

Two enzyme immunoassays (EIA) for trichinellosis in swine (a research version and a commercial kit obtained from LMD Agro-Vet) were used to test pig whole blood, serum, and tissue fluids as sample sources. Five pigs inoculated with 2,500 Trichinella spiralis were bled weekly and then sacrificed after 42 days. Blood and serum samples from each sampling time and tissue fluids collected at slaughter were tested in both EIA formats. Whether samples were undiluted or diluted, blood could be used as effectively as serum for detecting pigs infected with T. spiralis. Further, it was as effective to use tissue fluids as either blood or serum. With both tests, the research and commercial versions, it is possible to detect infected pigs by 28 days following infection. The results of this study suggest that a rapid EIA test such as the LMD Agro-Vet Trichinella Serology Microwell ELISA can be used as an effective tool for testing pigs for trichinellosis on the farm. Additionally, because it can enable the detection of antibodies in tissue fluids, this test could be used effectively to determine trichinellosis in meat samples following slaughter.

Keywords: EIA; PIGS; SEROLOGY; TRICHINELLA SPIRALIS

Document Type: Research Article

Affiliations: 1: U.S. Department of Agriculture, Agricultural Research Service, Parasite Biology and Epidemiology Laboratory, Beltsville, Maryland 20705, USA 2: American Romanian Technology Exchange, Bucharest, Romania

Publication date: November 1, 1996

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