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Comparison of Thin-Layer Chromatography and an Enzyme-Linked Immunosorbent Assay for Detection and Quantification of Deoxynivalenol in Corn and Wheat

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Abstract:

The trichothecene, deoxynivalenol (DON), is a cornmon mycotoxin found in wheat and corn. Detection methods for DON have limitations in accuracy, sensitivity, ease of use, and turn-around time. Two methods, thin-layer chromatography (TLC) and an enzyme-linked immunosorbant assay (ELISA) for DON detection and quantification were cornpared. The methods varied considerably in the amounts of DON detected in 51 different grain samples, with the ELISA method showing higher concentrations than the TLC method. Much of the unaccounted-for DON may have been lost during sample preparation for the TLC method. Recovery rates for the TLC method at DON levels of 1 and 5 ppm were 46% and 25% in corn grits and 32% and 26% in ground wheat respectively. Recovery rates for the ELISA method at 1 and 5 ppm levels were both 96% in corn grits and respectively 83% and 69% in ground wheat. The ELISA method was much faster and less laborious than the TLC method and did not use organic solvents, which are required in the TLC method.

Keywords: DEOXYNIVALENOL; DON; ELISA; TLC; VOMITOXIN

Document Type: Research Article

Affiliations: Department of Food Science and Technology, University of Nebraska at Lincoln, Lincoln, Nebraska 68583-0919, USA

Publication date: April 1, 1996

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    First published in 1937, the Journal of Food Protection®, is a refereed monthly publication. Each issue contains scientific research and authoritative review articles reporting on a variety of topics in food science pertaining to food safety and quality. The Journal is internationally recognized as the leading publication in the field of food microbiology with a readership exceeding 11,000 scientists from 70 countries. The Journal of Food Protection® is indexed in Index Medicus, Current Contents, BIOSIS, PubMed, Medline, and many others.

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