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Immunostick ELISA for Detection of Cow's Milk in Ewe's Milk and Cheese Using a Monoclonal Antibody against Bovine β-Casein

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Abstract:

An immunostick enzyme-linked immunosorbent assay (ELISA) has been developed for the rapid detection of cow's milk in ewe's milk or cheese. The assay uses a monoclonal antibody (AH4) produced against bovine β-casein for the detection of cow's milk or cheese bound to the paddles of immunostick tubes. This immunostick ELISA allows the visual identification of ewe's milk containing more than 1% of cow's milk or cheese samples containing more than 0.5% of cow's cheese.

Keywords: COW'S MILK DETECTION; ELISA; MONOCLONAL ANTIBODIES

Document Type: Research Article

Affiliations: Departamento de Nutrición y Bromatología III, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain

Publication date: April 1, 1996

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