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Campylobacter jejuni/coli: Methodology of Isolation and Possible Interfering Factors in Primary Culture

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The prevalence of Campylobacter jejuni and Campylobacter coli was investigated in 64 samples of fresh retail chicken purchased from commercial slaughterhouses located in Brazil. Campylobacter spp. were isolated from 40 (62.5%) of 64 analyzed samples. The strains biotyped according to Lior were classified as C. coli biotypes I and II, and C. cali biotypes I and II.

The efficiency of different procedures for recovering Campylobacter spp. from chicken carcasses was tested. The enrichment procedure was significantly less effective than direct plating (P < 0.05), detecting 19 of 40 (47.5%) as opposed to 38 of 40 (95%) positive samples. Using direct plating the efficiency of Blaser's selective supplement was significantly more effective (P < 0.05) than Skirrow's selective supplement. To verify which factors could be affecting Campylobacter spp. growth in enrichment broth, the pH was measured after incubation for 48 h at 42°C and lactobacilli, coliforms, and enterococci were enumerated.

Most of the Campylobacter-negative samples presented high levels of indicator microorganisms, which may have hindered the recovery of Campylobacter spp. during the enrichment procedure.


Document Type: Research Article

Affiliations: 1: Departamento de Tecnologia de Alimentos, Faculdade de Veterinária, Universidade Federal Fluminense, Brasil, CEP 21944-590; Rua Alvares de Azevedo no 40/1202, Icaraí, Niterói, Rio de Janeiro, Brasil, CEP 24220-021 2: Departamento de Tecnologia de Alimentos, Faculdade de Veterinária, Universidade Federal Fluminense, Brasil, CEP 21944-590 3: Departamento de Microbiologia Médica, Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brasil, CEP 21944-590

Publication date: April 1, 1996

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