Methods for the Detection of Trichinellosis in Horses

Authors: Gamble, H. Ray1; Gajadhar, Alvin H.2; Solomon, Morse B.3

Source: Journal of Food Protection®, Number 4, April 1996, pp. 341-440 , pp. 420-425(6)

Publisher: International Association for Food Protection

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Abstract:

Twelve horses were infected with various doses of Trichinella spiralis and then tested for infection using direct (artificial digestion) and indirect (enzyme immunoassay) methods. Horses became infected in a dose-dependent manner. Larvae accumulated preferentially in the tongue, followed by the masseter, neck, supraspinatus, trapezius, and diaphragm. At lower infection levels, the tongue harbored several times more parasites than were found in other tissues. The sensitivity of artificial digestion methods for detecting infections was directly related to sample size. One-gram samples were not reliable for detecting infection levels of <3 larvae per g (LPG). In sample sizes of 5 or 10 g the technique allowed infections as low as 1 LPG to be detected. The enzyme immunoassay (EIA) detected all infected horses; the times following infection at which horses became seropositive varied in a dose-dependent manner, but 11 of 12 horses were positive in the EIA by 4 weeks postinoculation. One horse, with a larval density in the tongue of 0.39 LPG, did not become seropositive until 7 weeks postinoculation. The results suggest that artificial digestion of horse carcasses for trichinae should concentrate on tissue samples from the tongue or masseter muscles. Sample sizes should be a minimum of 5 g using pooled-sample digestion methods to assure detection of all infections which might pose a human health risk. The EIA is a potential substitute for artificial digestion methods and could also be useful for antemortem testing and for epidemiological studies.

Keywords: ARTIFICIAL DIGESTION; EIA; HORSES; SEROLOGY; TRICHINELLA SPIRALIS

Document Type: Research Article

Affiliations: 1: USDA, Agricultural Research Service, Parasite Biology and Epidemiology Laboratory, Beltsville, Maryland 20705, USA 2: Agriculture and Agri-Food Canada, Health of Animals Laboratory, Saskatoon, Saskatchewan, Canada 3: USDA, Agricultural Research Service, Meat Science Research Laboratory, Beltsville, Maryland 20705, USA

Publication date: April 1, 1996

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    First published in 1937, the Journal of Food Protection®, is a refereed monthly publication. Each issue contains scientific research and authoritative review articles reporting on a variety of topics in food science pertaining to food safety and quality. The Journal is internationally recognized as the leading publication in the field of food microbiology with a readership exceeding 11,000 scientists from 70 countries. The Journal of Food Protection® is indexed in Index Medicus, Current Contents, BIOSIS, PubMed, Medline, and many others.

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