Inhibition of Growth of Mycotoxigenic Fusarium Species by Butylated Hydroxyanisole and/or Carvacrol
Abstract:The antifungal effect of butylated hydroxyanisole (BHA) and carvacrol alone and in combination on six toxigenic Fusarium species was examined in potato dextrose broth (PDB) cultures. The highest percentage of mycelial inhibition was recorded for F. poae in the presence of 200 μg/ml of BHA or carvacrol and BHA and carvacrol combined (50 μg/ml each and 100 μg/ml each), while F. moniliforme was the least sensitive of the test fungi at the same concentrations. A synergistic activity of BHA and carvacrol combined (50 μg/ml each) was observed against several of the test fungi. The pH of the filtrates from BHA or carvacrol and BHA plus carvacrol-amended cultures ranged between 5.0 and 5.4. The highest amount of total dissolved solids (TDS) was recorded for F. graminearum in the presence of 200 μg/ml of BHA. Leakage of amino acids, sugars, and protein was detected at each concentration of BHA or carvacrol alone and in combination. For the majority of the test fungi, the amount of leakage in the culture filtrates increased as the concentration of the test compounds increased. The leakage of sugars, amino acids, and protein may be an indication of mycelial memBHAne disruption by the BHA and/or carvacrol as an initial step in inhibition of the test fungi in vitro.
Document Type: Research Article
Affiliations: Health Research Center and Department of Biology, Southern University, Baton Rouge, Louisiana 70813, USA
Publication date: April 1, 1996
More about this publication?
- IAFP Members with personal subscriptions to JFP Online: To access full-text JFP or JMFT articles, you must sign-in in the upper-right corner using your Ingenta sign-in details (your IAFP Member Login does not apply to this website). The Journal of Food Protection (JFP) is a refereed monthly publication. Each issue contains scientific research and authoritative review articles reporting on a variety of topics in food science pertaining to food safety and quality. The Journal is internationally recognized as the leading publication in the field of food microbiology with a readership exceeding 11,000 scientists from 70 countries. The Journal of Food Protection is indexed in Index Medicus, Current Contents, BIOSIS, PubMed, Medline, and many others.
Print and online subscriptions are available to IAFP Members and institutional subscribers. IAFP Members with a subscription to JFP Online will have access to all available JFP and JMFT content. Online visitors who are not IAFP Members or journal subscribers will be charged on a pay-per-view basis. Membership and subscription information is available at www.foodprotection.org.
- Information for Authors
- Submit a Paper
- Subscribe to this Title
- Membership Information
- Information for Advertisers
- ingentaconnect is not responsible for the content or availability of external websites