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Simple and Rapid Method for Determination of Oxolinic Acid in Tiger Shrimp (Penaeus monodon) by High-Performance Liquid Chromatography

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A simple high-performance liquid chromatographic method for determining oxolinic acid in tiger shrimp (Penaeus monodon) tissue has been developed. The sample was simply extracted with ethyl acetate without further clean-up. The extract was analyzed using reversed-phase chromatography with UV detection at 330 nm. An extraction efficiency of 97.4 ± 10.3% was obtained. The within-run precision ranged from 1.46 to 4.21% and the between-run precision ranged from 6.01 to 9.77%. Detection of oxolinic acid in spiked shrimp was linear from 0.007 to 4 μg/g with a correlation coefficient of 0.998. The minimal detection limit of this procedure was 0.0035 μg/g.


Document Type: Research Article

Affiliations: 1: Department of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand 2: Department of Veterinary Public Health, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-21, Japan

Publication date: February 1, 1996

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