Authors: Lilly, Timothy¹; Solomon, Haim M.¹; Rhodehamel, E. Jeffery²

Source: Journal of Food Protection®, Volume 59, Number 1, January 1996 , pp. 59-61(3)

Publisher: International Association for Food Protection

Abstract:

Because modified atmosphere-packaged (MAP) vegetables may provide an anaerobic environment conducive to Clostridium botulinum growth and toxin production, the incidence of C. botulinum spores in commercially available, precut MAP vegetables was determined. One-pound (454-g) packages of MAP vegetables were aseptically opened, added to freshly steamed and cooled sterile trypticase-peptone-glucose-yeast extract broth and incubated at 35°C for 7 days. Positive and negative controls were included with each sampling. After incubation the broth cultures were tested for toxicity by the standard mouse bioassay. Of the 1,118 MAP vegetable packages examined, one package each of shredded cabbage, chopped green pepper, and Italian salad mix contained C. botulinum type A spores. One additional salad mix (main ingredient, escarole) contained both C. botulinum type A and type B spores. Results indicated a low overall incidence rate (0.36%) of C. botulinum spores in commercially available precut MAP vegetables.

Keywords: MODIFIED ATMOSPHERE PACKAGING; VEGETABLES; CLOSTRIDIUM BOTULINUM

Document Type: Research article

Affiliations: 1: Division of Microbiological Studies (HSF-516), U.S. Food and Drug Administration, 200 C Street, S.W., Washington, D.C. 20204, USA 2: Division of HACCP Programs, U.S. Food and Drug Administration, 200 C Street, S.W., Washington, D.C. 20204, USA

Publication date: 1996-01-01

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