Assay Procedures for Commercial Probiotic Cultures

Authors: Mccann, Tim1; Egan, Trish2; Weber, George H.1

Source: Journal of Food Protection®, Number 1, January 1996, pp. 6-107 , pp. 41-45(5)

Publisher: International Association for Food Protection

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Abstract:

A number of commercially available cultures, marked for human consumption, were analyzed for viability using a resuspension medium consisting of KH2PO4, Na2HPO4, cysteine, Tween 80, agar, and an antifoam agent together with modifications of multiple-layer diffusion techniques. These methods were compared to the use of MRS plus cysteine, acidified MRS, and M17 culture media and found to be superior in the selection and enumeration of dried cultures of mixed genera.

Keywords: ENUMERATION; PROBIOTIC; SELECTION; VIABILITY

Document Type: Research Article

Affiliations: 1: Georgetown Technology Group, 11240 S.W. Lynnvale Dr., Portland, Oregon 97225, USA 2: Food Products Laboratory, 12003 N.E. Ainsworth Circle, Suite 105, Portland, Oregon 97220-1099, USA

Publication date: January 1, 1996

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    First published in 1937, the Journal of Food Protection®, is a refereed monthly publication. Each issue contains scientific research and authoritative review articles reporting on a variety of topics in food science pertaining to food safety and quality. The Journal is internationally recognized as the leading publication in the field of food microbiology with a readership exceeding 11,000 scientists from 70 countries. The Journal of Food Protection® is indexed in Index Medicus, Current Contents, BIOSIS, PubMed, Medline, and many others.

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