Extraction of Aflatoxin from Peanut Meal and Kernels by CO2–Methanol Using a Highly Pressurized Fluid Extraction Process

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Abstract:

Peanut kernels infected with Aspergillus parasiticus after milling into meals were used as toxin-contaminated material for aflatoxin extraction using CO2–methanol. The percentage of aflatoxin recovery increased with CO2–60% methanol and decreased with CO2–l00% methanol with an increase in the methanol/peanut meal ratio. One gram of peanut meal and 6 ml of 60% methanol were deposited in an extraction chamber (ca. 13 ml), pressurized with CO2 to 3,000 psi at 50°C, and held for 15 min, followed by releasing and collecting the extraction medium. The extracted solution was applied to an affinity column for aflatoxin adsorption and to thin-layer chromatography for separation and fluorescence densitometry for component quantitation; an average of 97.6% of the aflatoxin was recovered. When this procedure and a commercialized immunochemical method were used for quantitation of aflatoxin in discolored peanut kernels collected from a commercial processing line, comparable results were observed.

Keywords: AFFINITY COLUMN; AFLATOXIN; PEANUTS; PRESSURIZED FLUID EXTRACTION

Document Type: Research Article

Affiliations: 1: Depanment of Food Engineering, Da Yeh Institute of Technology, Changhwa, Taiwan, Republic of China 2: Department of Food Industry, National Chiayi Institute of Agriculture, Chiayi, Taiwan, Republic of China

Publication date: July 1, 1995

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