Use of a Rapid Microbial ATP Bioluminescence Assay to Detect Contamination on Beef and Pork Carcasses

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Abstract:

A new microbial ATP bioluminescence assay was shown to be an accurate and rapid method to determine the levels of generic bacterial contamination on beef (n = 400) and pork (n = 320) carcasses sampled in commercial processing plants. Based on in vitro fecal dilution studies, the rapid microbial ATP (R-mATP) assay is as accurate as the standard plate count method for estimating bacteria in bovine or porcine fecal samples. The correlations (r) between the R-mATP assay and the standard aerobic plate count for beef and pork carcasses sampled in commercial processing were 0.91 and 0.93, respectively. A segmented-model statistical approach to determine the lower limits of assay sensitivity was developed. By using this model to analyze the in-plant data, the R-mATP test responded in a linear fashion to levels of microbial contamination of > log10 2.0 aerobic CFU/cm2 on beef carcasses and of > log10 3.2 aerobic CFU/cm2 for pork carcasses. The R-mATP assay requires approximately 5 min to complete, including sampling. Given the rapidity and accuracy of the assay, processors interested in monitoring critical control points in the slaughter process could potentially use the R-mATP assay to monitor microbiological prevention and intervention procedures for minimizing carcass contamination.

Keywords: ATP BIOLUMINESCENCE; HACCP MONITORING; RAPID MICROBIAL TESTING

Document Type: Research Article

Affiliations: United States Department of Agriculture, Agricultural Research Service, United States Meat Animal Research Center, P.O. Box 166, Clay Center, Nebraska 68933 USA

Publication date: July 1, 1995

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    First published in 1937, the Journal of Food Protection®, is a refereed monthly publication. Each issue contains scientific research and authoritative review articles reporting on a variety of topics in food science pertaining to food safety and quality. The Journal is internationally recognized as the leading publication in the field of food microbiology with a readership exceeding 11,000 scientists from 70 countries. The Journal of Food Protection® is indexed in Index Medicus, Current Contents, BIOSIS, PubMed, Medline, and many others.

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