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Typing of Epidemiologically Associated Environmental and Clinical Strains of Listeria monocytogenes by Random Amplification of Polymorphic DNA

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Abstract:

One hundred and three strains of Listeria monocytogenes(Lm), Listeria seeligeri and Listeria innocua of clinical, food, and environmental origin were examined by generating randomly amplified polymorphic DNA (RAPD). Using one 10 bp and two 13 bp random primers, epidemiologically related strains, previously shown to be indistinguishable by phage typing, yielded identical RAPD profiles. Strains isolated from the hands of three workers in a retail food establishment showed the presence of a single predominant Lm isolate. RAPD analysis is a rapid, reproducible, and relatively inexpensive method for the differentiation of epidemiologically linked isolates of Listeria spp.

Keywords: LISTERIA MONOCYTOGENES; PCR TYPING; RAPD

Document Type: Research Article

Affiliations: Department of Microbiology, University of Leeds, Old Medical School, Thoresby Place, Leeds, United Kingdom, LS2 9JT

Publication date: June 1, 1995

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