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Rapid Assessment of the Microbiological Quality of Poultry Carcasses Using ATP Bioluminescence

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The meat industry is in need of faster and more reliable methods to determine microbial loads in food products. A rapid method (<15 min) has been developed to assess the microbiological quality of chicken carcasses using the adenosine triphosphate (ATP) bioluminescence assay. The results indicate that, following modifications, the ATP bioluminescence test produced an acceptable correlation with plate counts (r = 0.85, p < 0.001) and demonstrated good repeatability between replicates. It is envisaged that the modified ATP bioluminescence assay would best be used as a platform rejection test. Using threshold levels determined from the regression equation, the ATP bioluminescence assays gave about 90% agreement with plate counts for carcass rinses with counts above 5 × 104 CFU/ml. These findings suggest that the modified ATP bioluminescence assay could be used for monitoring critical control points (CCPs) in programs based on hazard analysis of critical control points (HACCP).


Document Type: Research Article

Affiliations: 1: Food Science Department, University of Guelph, Guelph, Ontario N1G 2W1 Canada 2: Population Medicine, Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1 Canada 3: Health of Animals Laboratory, Agriculture Canada, 110 Stone Rd. West, Guelph, Ontario Canada N1G 3W4 4: Health of Animals Laboratory, Agriculture Canada, I 10 Stone Rd. West, Guelph, Ontario Canada N1G 3W4

Publication date: May 1, 1995

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