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Use of Differential Rumen Fluid-Based Carbohydrate Agar Media for Culturing Lactose-Selected Cecal Bacteria from Chickens

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Abstract:

A rumen fluid-based differential carbohydrate agar medium for enumerating chicken cecal carbohydrate-utilizing bacteria was tested. Eleven bacteria isolated from a continuous-flow culture that had been seeded with chicken cecal contents were inoculated on fifteen different media in an anaerobic environment. These media included various levels of rumen fluid (0%, 5%, 10%, 16%) and carbohydrate (glucose [GLU], galactose [GAL], lactose[LAC]). Viable cell recovery (colony-forming units [CFU]/direct cell counts × 100%) was higher for 16% rumen fluid-supplemented carbohydrate media than the lower concentrations of rumen fluid-supplemented carbohydrate media. In addition, average viable cell recovery of all 11 bacteria on differential carbohydrate media were significantly (P < 0.05) higher in galactose and lactose media than on the glucose media. Data indicated that 16% rumen-fluid based media can be used to enumerate anaerobic, lactose-fermenting subgroups of chicken cecal bacteria.

Keywords: CECAL BACTERIA; DIFFERENTIAL MEDIA; RUMEN FLUID

Document Type: Research Article

Affiliations: 1: Department of Poultry Science, Texas A&M University, College Station, Texas 77843 2: Department of Veterinary Pathobiology, Texas A&M University, College Station, Texas 77843 3: U. S. Department of Agriculture, Agricultural Research Service, Food Animal Protection Research Laboratory, Route 5, Box 810, College Station, Texas 77845

Publication date: April 1, 1995

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