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Recovery of Lactic Acid Bacteria on Petrifilm SM Under Various Incubation Atmospheres

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Abstract:

The ability of lactic cultures to grow on Petrifilm SM plates was studied. Frozen yogurt mix was analyzed microbiologically by plating on TOE, LBS, M17 and Petrifilm SM. Plates were incubated aerobically in a Oas-Pak System and under a CO2 environment. Also, Lactobacillus bulgaricus, Streptococcus thermophilus, Lactococcus lactis subsp. diacetylactis, and Leuconostoc cre-moris were isolated from yogurt and buttermilk samples for study. Isolated cultures were grown in nutrient broth and plated on Petrifilm SM and M17 agar. Plates were incubated aerobically and in a Oas-Pak system. Petrifilm SM plates performed as well or better than the M17 agar in assaying lactic growth with the exception of Streptococcus thermophilus culture in an aerobic environment. Petrifilm SM plates show promise as a method for enumerating viable lactic cultures if incubated in a reduced oxygen environment.

Keywords: ATMOSPHERE; LACTIC BACTERIA; PETRIFILMTM

Document Type: Research Article

Affiliations: Dairy Science Department, Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, Baton Rouge, Louisiana 70803

Publication date: March 1, 1995

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    First published in 1937, the Journal of Food Protection®, is a refereed monthly publication. Each issue contains scientific research and authoritative review articles reporting on a variety of topics in food science pertaining to food safety and quality. The Journal is internationally recognized as the leading publication in the field of food microbiology with a readership exceeding 11,000 scientists from 70 countries. The Journal of Food Protection® is indexed in Index Medicus, Current Contents, BIOSIS, PubMed, Medline, and many others.

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