Iron Supplementation to Enhance the Recovery of Salmonella enteritidis from Pools of Egg Contents
Abstract:Direct-plating culture methods have been proposed for use in programs to test eggs for contamination by Salmonella enteritidis (SE) because of their speed and low cost, but direct plating has previously been observed to detect SE less frequently than more elaborate broth-enrichment culture methods. The present study used experimentally inoculated pools of egg contents to assess the comparative sensitivities of direct-plating and broth-enrichment culturing for detecting SE, to evaluate the ability of iron supplementation to increase the multiplication of SE during incubation of egg pools, and to determine whether iron supplementation could enhance the ability of direct plating to detect SE in egg pools that initially contained very few SE cells. Efficient detection of SE in egg pools was found to require an approximately 10,000-fold higher level of SE for direct plating than for broth-enrichment culturing. Iron supplementation of contaminated egg pools significantly (P < 0.005) increased the resulting final level of SE after 1 day of incubation at 37°C. Iron supplementation also significantly (P < 0.005) increased the percentage of 10-egg pools, initially inoculated with fewer than 10 SE cells each, that were identified as contaminated by direct plating after the pools had been incubated for 1 day at 37°C. Increasing the iron availability in incubating egg pools, therefore, increased the probability that a small initial number of SE cells would grow quickly to levels likely to be detected by direct plating.
Document Type: Research Article
Affiliations: United States Department of Agriculture, Agricultural Research Service, Southeast Poultry Research Laboratory, 934 College Station Road, Athens, Georgia 30605
Publication date: March 1, 1995
More about this publication?
- IAFP Members with personal subscriptions to JFP Online: To access full-text JFP or JMFT articles, you must sign-in in the upper-right corner using your Ingenta sign-in details (your IAFP Member Login does not apply to this website). The Journal of Food Protection (JFP) is a refereed monthly publication. Each issue contains scientific research and authoritative review articles reporting on a variety of topics in food science pertaining to food safety and quality. The Journal is internationally recognized as the leading publication in the field of food microbiology with a readership exceeding 11,000 scientists from 70 countries. The Journal of Food Protection is indexed in Index Medicus, Current Contents, BIOSIS, PubMed, Medline, and many others.
Print and online subscriptions are available to IAFP Members and institutional subscribers. IAFP Members with a subscription to JFP Online will have access to all available JFP and JMFT content. Online visitors who are not IAFP Members or journal subscribers will be charged on a pay-per-view basis. Membership and subscription information is available at www.foodprotection.org.
- Information for Authors
- Submit a Paper
- Subscribe to this Title
- Membership Information
- Information for Advertisers
- Ingenta Connect is not responsible for the content or availability of external websites