Factors Affecting Aflatoxin B1 Removal by Flavobacterium aurantiacum
Abstract:This study was conducted to investigate several factors affecting the removal of aflatoxin B1 by Flavobacterium aurantiacum NRRL B-184. A simple spectrophotometric procedure was evaluated and compared to an established high-performance liquid chromatography (HPLC) method and found to be useful for determining aflatoxin concentration in test solutions of phosphate buffer. Using the spectrophotometric method, 72-h cultures of F. aurantiacum were observed to remove more toxin from solution than 24-h cultures. Likewise, populations of 1010 cells removed aflatoxin at a faster rate than did 109 cells, although the total amount removed did not differ. Transferring F. aurantiacum cultures in tryptic soy broth every 3 days for over 3 days for over 8 months had no apparent effect on their ability to remove measurable amounts of aflatoxin B1 from solution. Populations of 1 × 109 CFU/ml or less heat-inactivated F. aurantiacum were unable to remove aflatoxin B1 from phosphate buffer.
Document Type: Research Article
Affiliations: Center for Food Safety and Quality Enhancement, University of Georgia Experiment Station, Griffin, Georgia 30223-1797
Publication date: January 1, 1995
More about this publication?
- IAFP Members with personal subscriptions to JFP Online: To access full-text JFP or JMFT articles, you must sign-in in the upper-right corner using your Ingenta sign-in details (your IAFP Member Login does not apply to this website). The Journal of Food Protection (JFP) is a refereed monthly publication. Each issue contains scientific research and authoritative review articles reporting on a variety of topics in food science pertaining to food safety and quality. The Journal is internationally recognized as the leading publication in the field of food microbiology with a readership exceeding 11,000 scientists from 70 countries. The Journal of Food Protection is indexed in Index Medicus, Current Contents, BIOSIS, PubMed, Medline, and many others.
Print and online subscriptions are available to IAFP Members and institutional subscribers. IAFP Members with a subscription to JFP Online will have access to all available JFP and JMFT content. Online visitors who are not IAFP Members or journal subscribers will be charged on a pay-per-view basis. Membership and subscription information is available at www.foodprotection.org.
- Information for Authors
- Submit a Paper
- Subscribe to this Title
- Membership Information
- Information for Advertisers
- Ingenta Connect is not responsible for the content or availability of external websites