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The Development of a Quantitative ELISA for Aflatoxin B1 in Ground Peanuts Using Antibodies Isolated From the Yolk of a Laying Hen

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Abstract:

Antibodies directed against Aflatoxin B1 (AFB1) were produced in laying hens, isolated from egg yolk and applied in an enzyme-linked immunosorbent assay (ELISA) for AFB1 in ground peanuts. The ELISA sensitivity was improved by reduction of the amount of the coating antigen absorbed onto the microplate well surfaces. The main aflatoxins B1, B2, G1, G2, M1, aflatoxicol, sterigmatocystin were found to cross-react in the competitive ELISA, 100, 25, 23, 4, 0, 0, 0%, respectively. Aflatoxin B1 could be reproducibly detected and quantified in spiked ground peanuts at levels greater than 5 ppb using a hexane and methanol solvent based peanut extraction protocol.

Keywords: AFLATOXIN B1; ELISA; LAYING-HEN; PEANUTS; YOLK

Document Type: Research Article

Affiliations: 1: Department of Animal Science, University of Manitoba, Winnipeg, Manitoba, Canada, R3T 2N2 2: Department of Animal Science, University of Manitoba, Winnipeg, Manitoba, Canada, R3T 2N2; Department of Foods and Nutrition in the Faculty of Agriculture and Food Sciences, University of Manitoba, Winnipeg, Manitoba, Canada, R3T 2N2

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